APPLICATION OF ENZYME EXTRACTS OF BACTERIAL PECTINASE AND MANNANASEIN FABRIC STAIN REMOVAL

Abstract

Enzymes are capable of degradation of many substrates and can be used in many industrial applications. Degradative enzymes from bacterial origin are numerous and are employed in many industrial applications commercially. Detergent formulations need enzymes that can degrade stains containing substrates like protein, pectin, sticky gum, etc. Present study describes the application of pectinase (Pectin degrading enzymes) and mannanase (mannans or gum degrading enzymes) enzyme purified from bacterial species of hot spring origin. Enzymes purified to homogeneity from bacterial grown culture and found to have activity 79.37 U/ml of mannanase and 299 U/ml of pectinase. Both enzymes were highly stable in tested inactivated commercial detergent solution retaining considerable catalytic activity. Their cleaning action was effective in getting rid of stains with and without detergents. Morphological and cultural studies have identified the mannanase producing bacteria as Bacillus licheniformis and pectinase producing species belonging to Anoxybacillus genus.