A Biochemical Approach to Understand the Concept of Snigdha Guna of Ghee and Ruksha Guna of Terminalia arjuna on Behaviour of 3T3-L1 Cell Line with Respect to Adipocytic and Anti-Adipocytic Activity : In Vitro

  • Ruchika Dighekar Assistant Professor, Department of Kriya Sharira, Sunil Ramsing Chunawale Ayurved College, Chikhali,
  • Kiran Tawalare Assistant Professor, Department of Kriya Sharira, Shri Ayurved College, Nagpur, Maharashtra, INDIA.
Keywords: Basic Principles of Ayurveda, Gurvadi Guna, Snigdha Guna, Ruksha Guna, 3T3-L1 cell line.


Ayurvedic medicine is a system of traditional medicine native to India. Ayurveda is based on peculiar fundamental principles like Shatpadarth, Tridosha Theory, Panchamahabhuta Theory, Prakriti, Ojas, Dhatu, Mala, Agni, Manas, and Atma etc. The concepts of Ayurveda are expressed with Gunas. The increased elements are treated by opposite Guna. In support of the above concept, an experimental study on the 3T3-L1 cell line is carried out for examine the adipocytic and anti adipocytic activity of Snigdha and Ruksha Guna respectively. The results were assessed with the help of evaluation of sublethal concentration by using XTT assay and lipid assay. The aim of basic research in Ayurveda through bioscience is to explore scientific innovation and opportunities in fundamental concept of Ayurveda. Fundamental research needs to be done to highlight the concept of gurvadi guna through the in vitro study.